Esame delle urine: si cambia Published in: La Rivista Italiana della Medicina di Laboratorio, July 2017 DOI: 10.1007/s13631-017-0157-7: Authors: Rudi Ravasio, Giulia Previtali, Francesca Nembrini, Maria Grazia Alessio View on publisher site Alert me about new mentions. Specific gravity (refractometry based) and clarity are measured in a flow cell, and color is rated with a specific algorithm against the blank pad on the test strip. Mattenheimer H, Adams EC. The dashed lines represent the lower limit of detection (− − −) with the 2 SD limits (- - - -). Viene venduto in scatole da 25 pezzi. 0000017558 00000 n Urine per esami di citologia urinaria raccolte Castelfranco Emilia. SPERMIOGRAMMA. ESAME URINE PER IDONEITA’ AGONISTICA MEDICINA DELLO SPORT ... Consegnare il presente modulo e la provetta con le urine (almeno 4 giorni lavorativi prima della data della visita medico sportiva ) presso uno dei seguenti Centri Prelievo, nei giorni e negli orari indicati. stream Secrezioni mammarie. In recent studies, the correlation (r) between UFC WBCs and WBCs by counting chamber was 0.93–0.98, and that of UFC RBCs and RBCs by counting chamber was 0.83–0.89 (10)(25)(26). Modificato il 18 aprile 2017 . Access to the instrument’s raw data theoretically allows a higher analytical sensitivity for several analytes. Viberti GC, Jarrett RJ, Wiseman MJ. COME RACCOGLIERE LE URINE? L’esame di analisi delle urine (ECMU: Esame Chimico Morfologico Urine) è un comune test di screening, non invasivo per il paziente poiché è necessario un campione delle urine che dev’essere raccolto in un contenitore sterile per la raccolta delle urine. 1. Particles that cannot be classified in one of the former categories are counted as “other cells”. Alkalinization of urine gives rise to erroneous results in particle counting. Oxford University Press is a department of the University of Oxford. For WBCs, we found a lower limit of detection of 19 × 106 cells/L (CV = 1.2%). Ottomano C. Tecnologia ed automazione dell’ esame urine quantitativo. • Apporre il timbro del C. Prelievi presso cui è stato accettato il campione. We investigated the possible use of these meters to complement flow cytometry of urine and compared reflectance readings with quantitative determinations of urinary glucose and microalbumin. When urinary WBC counts were higher than the upper reference limit (25 × 106 cells/L), the following regression equation was obtained: y (%) = 83.7 −15.4 logx (106 cells/L); Spearman r = −0.688; P <0.001. Per la raccolta delle urine delle 24 ore è necessario seguire scrupolosamente questa procedura: Il mattino del giorno d'inizio raccolta, scartare tutta l'urina della prima minzione mattutina, svuotando completamente la vescica nel WC. Winkens RAG, Leffers P, Degenaar CP, Houben AW. Quantitative determination of hemoglobin in urine. The protein test (n = 220) detected 86% (95% confidence interval, 78–92%) of samples with <30 mg/L albumin with a specificity of 84% (95% confidence interval, 76–91%). Urine puternic acide (pH < 4,5) se produc in procesele maligne (are loc distrugerea crescuta de proteine), febra, diaree abundenta, acidoza diabetica sau metabolica. Citologia per espettorato. Reproducibility of protein, leukocyte esterase, hemoglobin, and glucose on the URISYS 2400 analyzer. This could improve level 1 urinary screening (12): it offers an improved, fast, reliable method that is easy to handle and usable in primary-care laboratories. Colombo JP eds. • Applicare a lato un’etichetta con il codice a barre. As is the case for test strips in general, the method is based on the so-called “indicator error” principle, in which proton exchange between the indicator on the strip and the proteins in the solution produces a color change from yellow to green-blue. The principle is based on argon laser flow cytometry. Ce intelegem din rezultatul examenului de urina? The UF-100 automatically detects and counts red blood cells (RBCs), white blood cells (WBCs), bacteria, yeast cells, crystals, epithelial cells, small round cells, sperm cells, and casts. The within- and between-run CVs for protein, leukocyte esterase, hemoglobin, and glucose are summarized in Table 1. 0000016774 00000 n Examenul de urina este un test de diagnostic si ajuta la identificarea substantelor si a materialului celular din urina, asociate cu diferite tulburari metabolice si renale. Peele J, Gadsden R, Crews R. Semi-automated vs. visual reading of urinalysis dipsticks. 3. A lower limit of detection of 8 × 106 cells/L (CV = 1.2%) was calculated. %�쏢 1. 2. Moreover, a 16% false-positive rate may be unacceptable for screening. Hence, extremely alkaline urine samples were not present in the samples we investigated. Predicting diabetic nephropathy [Letter]. (Insieme con il test viene fornito il contenitore urina da 90ml e un modulo in triplice copia).Questo Test Antidroga ha un sistema di stampa del risultato tramite il suo Strumento con software, oppure il risultato del test può essere anche scansionato o fotocopiato. 3. 0000016556 00000 n ISTRUZIONI PER LA RACCOLTA DELLE URINE DURANTE LE 24 ORE Il mattino del giorno precedente l’esame svuotare la vescica alla solita ora (per esempio alle ore 07.00) e, da quel momento in poi, raccogliere tutte le urine successive della giornata e della notte dell’esame fino alle ore 07.00 comprese; Kutter D. The urine test strip of the future. Regeniter A, Haenni V, Risch L, Köchli HP, Colombo JP, Frei R. Urine analysis performed by flow cytometry: reference range determination and comparison to morphological findings, dipstick chemistry and bacterial culture results – a multicenter study. Leukocyte esterase reflectance (y; %) = 83.7 − 15.4 log(WBC count; 106 cells/L); Spearman r = −0.688; P <0.001. The detection limit of the URISYS 2400 protein assay was 25 mg/L if restricted to albumin alone and total protein was not considered. Because urinalysis test strips are often used for checking urinary flow cytometry data (11), there is a need for a more quantitative evaluation of urinalysis test strips. Modde H, Rodriguez S. Urin Mikrobiologie. endobj Combining diagnostic information provided by urinary flow cytometry and more quantitative test strip analysis therefore offers a theoretical basis for the development of diagnostic expert systems (11)(12). TEST DI MEARES. Ben-Ezra J, Bork L, McPherson RA. È un’analisi che valuta caratteristiche fisiche come colore, aspetto, odore, ma anche alcune componenti chimiche come proteine, sangue e glucosio. Appunti per la prova scritta ed orale dell’esame di inglese, modulo 1, 2 e 3 previsto dal nuovo ordinamento per la facoltà di medicina e chirurgia per gli studenti del triennio biologico. These test strips are the same as used in the Miditron but are supplied in a cassette holding 400 test strips for leukocyte esterase, nitrite, protein, glucose, ketones, urobilinogen, bilirubin, hemoglobin/myoglobin, and pH. http://www.westgard.com/lesson29.htm (Accessed May 2002).. Froom P, Bieganiec B, Ehrenrich Z, Barak M. Stability of common analytes in urine refrigerated for 24 h before automated analysis by test strips. When monitoring our samples, we found a median pH of 6.5 (95% interval, 5–8). It has been extensively evaluated for urinalysis (9)(10)(11)(15) as well as for cerebrospinal fluid analysis (16). Campione richiesto: urina fresca raccolta al mattino, dopo aver scartata quella della notte. 2. Serum free light chain analysis and urine immunofixation electrophoresis in patients with multiple myeloma Autori: Nowrousian MR, Brandhorst D, Sammet C et al. <> Najčešće hemijske analize su analize na proteine, šećer, ketonska tela, urobilinogen, bilirubin i nitrite. Similarly, protein reflectance data and total protein measurements correlated well (Spearman r = −0.921; P <0.001), which is expected as long as the major protein is albumin. Mendeley readers. Screening school children for albuminuria, proteinuria and occult blood with dipsticks. Results: Reproducibility of the reflectance signal was good for high- and low-concentration urine pools for protein (0.8% and 0.9% and 1.5% and 2.2% within and between runs, respectively), leukocyte esterase (1.1% and 1.0%; 5.1% and 1.2%), hemoglobin (1.7% and 1.1%; 8.9% and 1.1%) and glucose (2.1% and 0.5%; 6.5% and 2.3%). 0000017589 00000 n This implies that the test has the potential to offer a screening for microalbuminuria without increased cost, hence coming closer to the “urine test strip of the future” (23). Visualizza la pagina con l'elenco dei punti prelievo aggiornata. Modulo di richiesta: deve riportare Nome, Cognome, data di nascita e codice fiscale, struttura e/o Medico richiedente nonché i dati clinici essenziali (ematuria, litiasi, precedenti neoplasie vescicali, urine da catetere, da ureterostomia, da vescica ileale).. Modulo Esame Citologico. Correlation between glucose measurements (glucose concentration different from 0) by the hexokinase-based method and glucose test strip reading (n = 85). Dilution was also monitored with a range of specific gravity of 1.005–1.033 and a median result of 1.017. Higher correlations were observed for biochemical and test strip data comparing protein and albumin (r = −0.825) and glucose data (r = −0.851). This instrument offers the possibility to obtain reflectance readings. endobj The correlation coefficient for the glucose signal with the routine hexokinase-based method was −0.851. L’esame delle urine è tra le prime indagini richieste per la valutazione della funzione renale. The following regression equation was obtained: y (%) = 78.2 −19.4 logx (106 cells/L); Spearman r = −0.680; P <0.001; Fig. S. C. Medicina di Laboratorio MODULO RACCOLTA URINE 24 ORE Revisione n.0 del 27/08/2017 PROCEDURE PER LA RACCOLTA DELLE URINE NELLE 24 ORE 1. xref Mattenheimer H, Adams EC. European urinalysis guidelines. L’analisi del sedimento urinario valuta inoltre la presenza di cellule, cristalli e cilindri. On the basis of ROC analysis, diagnostic sensitivity was 86% (95% confidence interval, 78–92%) and specificity was 84% (95% confidence interval, 76–91%) for a reflectance value of 55.6% when the nephelometric assay was used as the comparison method at a cutoff of 30 mg/L (n = 220; 113 positive and 107 negative cases, respectively). 0000017717 00000 n Van Acker J, Delanghe J, Langlois M, Taes Y, De Buyzere M, Verstraete G. Automated flow cytometric analysis of cerebrospinal fluid. The presence of haptoglobin in urine enhances the peroxidase activity of hemoglobin (29)(30). The reproducibility of (semi)automated readings is at least as good as visual readings (2), but most authors find the analytical, clinical, and labor cost-saving advantages of (semi)automated vs visual reading to be obvious (3). We found a close correlation between the log-transformed albumin results (x) and the test strip reflectance results (y): y (%) = 68.2 − 9.26[logx (mg/L)]; Spearman r = −0.825; P <0.001; Fig. Recently, the URISYS 2400 automated urine test strip analyzer (Roche Diagnostics) was introduced. Agreement between automated flow cytometry and test strip data was evaluated by Spearman rank analysis. The correlation between WBC data (x) and leukocyte esterase measurements (y) is shown in Fig. 0000000752 00000 n Correlation between RBC counts (x; flow cytometry) and test strip hemoglobin concentration (y) for RBC counts >25 × 106 cells/L (n = 96). Because microalbuminuria is characterized by large intraindividual variability, an ordinal-scale answer might be misleading. Regression equation for urinary WBC counts above the upper reference limit (25 × 106 cells/L; n = 132). delegato presso un qualunque centro prelievi Test strip measurements had reasonable lower limits of detection for WBC (19 × 106/L). 0000016709 00000 n The intensity of the reaction color of the test pad is detected by measuring the percentage of light reflected from the surface of the test pad. Od krvi se razlikuje po tem, da ne vsebuje krvnih celic (eritrocitov, levkocitov, trombocitov) in makromolekularnih snovi – beljakovin. It is known that reducing substances (e.g., ascorbic acid) may lower the signal, whereas oxidizing substances may have a positive effect on measured hemoglobin concentration. In contrast, conductivity showed a t-value of only 1.477 (P = 0.141). The reproducibility of (semi)automated readings is at least as good as visual readings (2), but most authors find the analytical, clinical, and labor cost-saving advantages of (semi)automated vs visual reading to be obvious (3). In this study, we wanted to investigate the performance of quantitative urinary test strip analysis. 17 0 obj Raccogliere le prime urine del mattino o, in alternativa, le urine emesse dopo almeno 2 ore dalla prima. Anno: 2005 Modulo: 6 Identification of amyloidogenetic light chains requires the combination of serum-free light chain assay with immunofixation of serum and urine Urine per esami di citologia urinaria Sassuolo. The strips include reagent pads for ordinal scale reporting of relative density, pH, leukocyte esterase, nitrite, protein, glucose, ketones, urobilinogen, bilirubin, and hemoglobin/myoglobin. Two discrepancies (0.9%) were found in which the test strips on both automated strip readers overestimated the urinary albumin concentration by >10-fold. The lower limit of detection was 25 mg/L (CV = 2.2%) (18). Gambke B, Kouri T, Kutter D, Nagel D, Schmid R, Scholer A. Miditron® multicenter evaluation. Does antihypertensive therapy protect the kidney in essential hypertension?. Contenitore pulito (es. Valori scazute ale pH-ului urinar (deci urine acide) se intalnesc in: Reumatismul poliarticular cronic; startxref Barrare la sede nella quale si effettua la visita Via Alessandrini, 6 Consegnare il presente modulo e la provetta con le urine (almeno 4 giorni lavorativi prima della visita) presso uno dei seguenti Centri Prelievo nei giorni e negli orari indicati. Ultimo aggiornamento: 19 Luglio 2019 Condividi. Sacks DB, Bruns DE, Goldstein DE, Maclaren NK, McDonald JM, Parrott M. Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus. • Inserire il codice esame (ECU ) e il codice richiedente 6650. The presence of ascorbate oxidase on the glucose test field prevents interference by ascorbic acid. It could be of special interest in patients with undiagnosed diabetes or hypertension in whom microalbuminuria is regarded as an excellent marker for assessing early renal damage (4)(5)(6)(7), particularly because many patients with non-insulin-dependent diabetes mellitus are asymptomatic and their diabetic state remains undiagnosed for years (24). Title: Modulo Es Urine Unificato As shown by our multiple regression model and dilution experiments, the hemoglobin field reflectance test is not influenced by dilution effects. The two outer solid lines represent the 95% prediction interval around the regression line. The UF-100 flow cytometer automatically determines the conductivity, so we performed multiple regression analysis on the study population, calculated in a regression model the hemoglobin reflectance vs RBCs and conductivity, and found that the t-value (as expected) for RBCs was −8.442 (P <0.0001). Additional dilution experiments were carried out. Nissell H, Kublickas M, Lunnell NO, Petterson E. Renal function in gravidas with chronic hypertension with and without superimposed preeclampsia. The two outer solid lines represent the 95% prediction interval around the regression line. >> 16 0 obj 14 22 La documentazione di riferimento per l‟esecuzione dell‟esame urine dovrebbe comprendere una accurata descrizione della fase preanalitica, analitica e post-analitica. Controlla la creatinina e Ph. 0000001055 00000 n The role of automated urine particle flow cytometry in clinical practice. ����,����bdm,�1"�Ql�m�[2ƥ+����M����}1��a4�Wrg��c�qE:�7輬M�-sx���L�+%{���^�" J�ك�M�Nm0�0�t���=Ƴ)׿1��?���L. MODULO DI RICHIESTA ESAMI CITOLOGIA ED ISTOLOGIA via Ettore Majorana 10 - 25020 Flero (BS) - T. 030.6950238 - F. 030.6950239 - P.IVA 03005430982 CITOLOGIA [ ] Esame Citologico Singola Lesione 3034 [ ] Esame Citologico Midollo + Emocromo 3035 [ ] Esame Citologico LCR + Chimico Fisico 3036 [ ] Esame Citologico Liquido Da Versamento 3037 The hemoglobin measurement is based on the peroxidase principle. The ratio of hemoglobin reflectance to RBC count was constant when osmolality was >190 mosmol/L. Per informazioni e/o chiarimenti rivolgersi a : segreteria U.O. This is stable for 30 days when stored tightly capped at 2–8 °C. Because microalbuminuria is generally regarded as an excellent marker for assessing early renal damage in common conditions such as diabetes and hypertension (4)(5)(6)(7) and as an early predictor of preeclampsia during pregnancy (8), the availability of highly sensitive test strip readers opens interesting perspectives for assessing this phenomenon. Multiple regression analysis on the study population showed no effect of conductivity on test strip leukocyte esterase. P values <0.05 were considered significant. 0000016924 00000 n L'esame delle urine richiesto per la certificazione di idoneità sportiva agonistica deve essere effettuato prima della data della visita (entro i 30 giorni precedenti) utilizzando l'apposito modulo fornito dalla Società Sportiva per l'accesso al laboratorio analisi e seguendo le istruzioni. 14 0 obj BREATH TEST. Opis fizičkih i fizičko-hemijskih karakteristika mokraće: izgled, boja, miris, reakcija i specifična težina. The inhibitory effect of urine on the peroxidase-like activity of hemoglobin and on horseradish peroxidase. Whelan PV, McGill C, Ng K. Evaluation of the BMC Chemstrip urine analyzer and Chemstrip 10 UA test strip with emphasis on comparison with analytical, chemical, physical, and microscopic methods [Abstract]. Istruzioni per esecuzione esame urine per idoneità sportiva agonistica Compilare il modulo sotto allegato necessario per l'accettazione del campione di urina . 0000016642 00000 n The dashed lines represent the lower limit of detection (− − −) with the 2 SD limits (- - - -). The change from urine microscopy to urinary flow cytometry has been accompanied by a significant decrease in imprecision (15). 0 Test strip urinalysis was carried out before flow cytometry analysis (Sysmex UF-100; TOA Medical Electronics), using URISYS strips on a URISYS 2400 analyzer (Roche Diagnostics). Analiza da indicii despre prezenta infectiilor urinare, sangerarilor la nivelul sistemului urinar, a calculilor urinari sau a anumitor boli ale sangelui, cum ar fi diabetul sau hepatita. Citologia nasale. urine delle successive 24 ore, comprese quelle delle ore 7.00 del mattino successivo; durante tutto il periodo di raccolta mantenere il contenitore chiuso in luogo fresco e su superficie sicura, rigorosamente al buio e avvolto in carta stagnola, lontano dalla portata This offers several possibilities: (a) The sensitivity for albumin may allow affordable screening for microalbuminuria, particularly in patients with undiagnosed renal damage. 0000000868 00000 n Background: Urine test strip results are generally reported in categories (i.e., ordinal scaled), but automated strip readers are now available that can report quantitative data. Ins Pagina 8 Bonnardeaux A, Somerville P, Kaye M. A study on the reliability of dipstick urinalysis. Bacterial peroxidases can also contribute to total peroxidase activity in urine (23). It must be noted, however, that the sensitivity (86%) may be inadequate for patients with known diabetes in whom physicians do not want to miss microalbuminuria in their annual testing. x��]��q�\t�I�W�(v�q.�'{G��|�#�*�|I��V*%�4��HJ�$W����0 z�=�K�,�'K%���h�t����~�dT2�q��nrƪ�����?>�N�dn�~�����9Nn�n���31�u����� s0��f�j�}��0o��ٹ�vV:L�n�nw�~t9G/�}�-��?���zk��v����e���;�9����~�Q�x���K�#�q�xf�����˃&��x�h]�w��vJ�>Z�ڇ�B~Wٺ��7����fZ�]��U�2�� ��n�n� 0000017620 00000 n Examen sumar de urină Ce este examenul sumar de urină? Because of the procedure for applying the urine to the test strips on the URISYS 2400, improper dipping is no longer a problem, nor is confusion about sample identification or urine contamination caused by dipping the strip in the tube, which potentially leads to interferences with chromatographic methods (31). The lower limits of detection for erythrocytes and leukocytes were 8 × 106/L and 19 × 106/L, respectively. Method validation-the detection limit experiment. 0000007901 00000 n endobj ����~��ݟ_��3��?��s!b��c�x���S涴{���5�֓῰���ĭ#��B}��W������9���,��@Kw_9[g!�s�^�~m~=r��/�1ɸw�ֺ4�koK1��W!ň��8��DI99��� ��-t��,$н�3�_�X p� �Nd�zN���8�7����4�S�xS}�{Gi`E���s1k�� Data are expressed in an ordinal scale (as “normal”, “negative”, “positive”, or as nominal concentrations) on the reports, but (quantitative) reflectance data can be downloaded to floppy disks. bicchiere di plastica monouso). Provetta a fondo conico, trasparente, fornita dal Laboratorio/Centro Prelievi o acquistata in Farmacia. Multiple regression analysis was used to investigate a model relating leukocyte esterase and hemoglobin field reflectance. (b) In addition, hemoglobin and leukocyte esterase reflectance data are useful for verifying flow cytometric data on RBCs and WBCs. Nulla è dovuto a pagamento dell’esame urine se l’interessato è minorenne o disabile : se maggiorenne, il pagamento di tale esame è compreso nell’importo dovuto per la visita. Istruzioni per la raccolta delle urine per l'esame delle 24 ore. %PDF-1.4 Because microalbuminuria is defined as excretion of 30–300 mg of albumin/24 h (20–200 μg/min, or 30–300 μg/mg of creatinine) (20), the protein field result allows identification of microalbuminuria cases in contrast to the classic reading and reporting of the strips, which can detect only albumin concentrations 150–200 mg/L or higher (21)(22). Scaderi anormale. Modulo di prenotazione. We also found good agreement between the hexokinase-based glucose method (x) and glucose test strip reflectance reading (y): y (%) = 53.9 − 19.3 logx (mmol/L); Spearman r = −0.851; P <0.001. Prendere nota dell'ora (ad esempio ore 7). The dashed lines represent the lower limit of detection (− − −) with the 2 SD limits (- - - -). To fully explore the possibilities of the albumin test pad in first-line diagnosis, a formal study should be performed. In conclusion, quantitative urine test strip analysis provides reliable data on WBCs, RBCs, glucose, and albumin. This leads to improved elimination of occasional errors in the WBC and RBC counting channels of the flow cytometer (11). The imprecision of urinary flow cytometry is far less than that of conventional urinary microscopy, but in some cases urinary flow cytometry reports erroneous results because of analytical interferences (e.g., calcium carbonate crystals falsely increase erythrocyte counts; confusion between yeast cells and erythrocytes). Test results, therefore, no longer need to be expressed in an ordinal scale. trailer Ce putem intelege din rezultatul acestei analize medicale: Nu este nici o problema grava acolo. 0000000015 00000 n 0000028490 00000 n Kouri T, Kähkönen U, Malminiemi K, Vuento R, Rowan M. Evaluation of Sysmex UF-100 urine flow cytometer vs chamber counting of supravitally stained specimens and conventional bacterial cultures. In particular, we wanted to compare the reflectance readings of the protein test field with immunochemical microalbumin determinations. Urine test strip analysis was performed with the automated URISYS 2400. Glucose reflectance (y; %) = 53.9 − 19.3 log(glucose; mmol/L); Spearman r = −0.851; P <0.001). <> The reproducibility of urinalysis using multiple reagent test strips. TAMPONI URETRALI. Urin je ultrafiltrat plazme in ga tvorijo ledvice. La provetta contenente le prime urine della mattina deve essere portata e consegnata dall'utente o da suo? The results are displayed in scattergrams, histograms, and as counts per microliter as well as counts per high-power field. 4 depicts the correlation between both measurement methods and the lower limit of detection of 0.17 mmol/L (CV = 1.3%) with the 2 SD limits. Because RBCs tend to lyse in urine, we investigated the linearity of the hemoglobin test strip pad. Albumin in urine was measured immunonephelometrically on 220 randomly selected samples with use of commercially available Behring antibodies on a Behring Nephelometer II analyzer (Dade Behring) standardized against the widely accepted WHO/College of American Pathologists Certified Reference Material 470. Search for other works by this author on: Author for correspondence. ESAME CITOLOGICO URINE Modulo da far compilare al paziente al momento della consegna dei campioni entile Signore/Signora, la disponibilità di alcune notizie cliniche è di grande aiuto per una corretta valutazione dell’indagine richiesta. mikroskopski pregled taloga koji se … Fenili D, Pirovano B. Bennett PH, Haffner S, Kasiske BL, Keane WF, Mogensen CE, Parving HH, et al. Langlois et al. The dashed lines represent the lower limit of detection (− − −) with the 2 SD limits (- - - -). Examen de urina Pagina 8 - Reducere a capacitatii rinichilor de a asigura filtrarea si eliminarea produselor de rebut ale sangelui, de a controla echilibrul corpului in apa si saruri si de a regulariza presiunea sangvina. The two outer solid lines represent the 95% prediction interval around the regression line. The two outer solid lines represent the 95% prediction interval around the regression line.

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